Interferongamma or IFNγ is a cytokine which plays a significant role in causing and regulating a variety of immune reactions. Cellular responses to IFNγ are mediated with its heterodimeric cellsurface receptor which triggers downstream signal transduction cascades, finally resulting in the regulation of gene expression.
To be able to study the function of IFNγ in several immune pathways and responses, researchers have developed mice with varied patterns of IFNγR receptor expression. The Individual Interferon-gamma ELISA Kit is designed for the quantitative measurement of individual IFN-γ in biological fluids such as serum, plasma, and cell culture supernatants. You can know more this kit through Boster Bio.
IFN-γ is mainly secreted by T cells and NK cells, but can also be generated by dendritic cells and macrophages in smaller quantities. IFN-γ is a pleiotropic cytokine that modulates a wide range of immunological functions including antimicrobial and antitumor action, and increases the surface expression of MHC class I and II proteins.
The assay relies on the sandwich ELISA method, where samples have been added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The recorded cytokine is detected by means of a biotinylated detection antibody, followed closely by streptavidin-horseradish peroxidase, which contrasts the biotinylated antibody.
IFN gamma is a macrophage activation factor, and immune interferon that's produced chiefly by T-lymphocytes and natural killer cells in response to antigens, mitogens along with other cytokines. IFN gamma is a dimeric protein comprising 2 146 amino acid subunits. IFN gamma is involved in cytokine regulation and acts synergistically with other cytokines.